Antigen-specific T cell activation requires interaction of the T cell with Specialized antigenpresenting cells. Signaling through the TCR is necessary but not sufficient to induce antigenspecific T cell activation and cytokine secretion. This
first
signal, termed signal 1, is both antigenspecific and MHC-restricted. Signal 2, wich is neither antigen-specific nor MHC-restricted, is necessary to induce cytoine secretion, cellular proliferation, and effector function. Recently immunological
studies
in T cell activation area are mainly focused on biological and molecular biological characterization of TCR-CD3 complex and accessary molecules providing costimulatory signal (signal 2). If signal 2 is not delivered, T cell enter a state of long
term
unreponsiveness to specific antigen-termed anergy. Monoclonal antibody technique has been especially involved in recognizing novel inducible cell surface antigens on T cell activation. This study was aimed to develop monoclonal antibodies
recognizing
novel cytoplasmic proteins present in activated T cells. We make 6 monoclones involved in changing pattern of T cell activated cytoplasmic proteins. Using these 6 monoclonal antibodies analyze to find novel molecules involved in T cell activation
associated response, apoptosis, and/or heat shock response of the T cells in early T cell activation.
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